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The retention variable is calculated by multiplying the distribution regular by the volume of stationary phase within the column and dividing by the volume of cellular phase while in the column.Exactly where N is the quantity of theoretical plates, L would be the size of your column, and H is the height equivalent to a theoretical plate. The plate
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Personal compounds are determined by an correct detector soon after exiting the column and transmitted being a sign to the computer’s HPLC software program.You could possibly as a result claim that non-polar molecules were extra soluble within the hydrocarbon about the surface area in the silica than They can be during the polar solvent - and so